Performances of Dried Blood Spots and Point-of-Care Devices to Identify Virological Failure in HIV-Infected Patients: A Systematic Review and Meta-Analysis.

To broaden access to HIV viral load monitoring (VLM), the use of blood samples from dried blood spots (DBS) or point-of-care (POC) devices, could be of great help in settings where plasma is not easily accessible. The variety of assays available makes the choice complex. This systematic review and meta-analysis aims to estimate the sensitivity and specificity of DBS and POC devices to identify patients in virological failure using World Health Organization (WHO) recommendations (viral load ≥1000 copies/mL), compared with plasma, for the assays currently available. Four databases were searched for articles, and two reviewers independently identified articles reporting sensitivity and specificity of DBS and/or POC to identify patients in virological failure. We excluded articles that used other thresholds as well as articles with a total number of participants below 50 to avoid reporting bias. Heterogeneity and factors associated with assays' performances were assessed by I2 statistics and metaregression. The protocol of this review follows the PRISMA guidelines. Out of 941 articles, 47 were included: 32 DBS evaluations and 16 POC evaluations. Overall, when using DBS, the Abbott RT HIV-1, Roche CAP-CTM, NucliSENS BioMerieux and Aptima assays presented sensitivity and specificity exceeding 85%, but reported results were highly heterogeneous. Factors associated with better performances were high volume of blood and the use of the same assay for DBS and plasma VLM. Regarding the POC devices, SAMBA I, SAMBA II, and GeneXpert devices presented high sensitivity and specificity exceeding 90%, with less heterogeneity. DBS is suitable VLM, but performances can vary greatly depending on the protocols, and should be performed in trained centers. POC is suitable for VLM with less risk of heterogeneity but is more intensive in costs and logistics.

Digital transformation in higher education from online learning perspective: A comparative study of Singapore and Vietnam

Digital transformation has been inevitable in all socio-economic fields, including higher education. Recently, under the burden of the COVID-19 pandemic, many universities have to change their entire teaching systems to online learning to ensure their students' learning is not interrupted. Thus, it is essential to study how universities' students, educators, and administrators perceive online learning in different countries. To this aim, this study investigates the factors affecting university members' preference for online learning in Singapore and Vietnam. Using a cross-country sample with a sound theoretical framework of the Technology Acceptance Model (TAM), we found that each member group in the university was influenced by a different weight of factors. Specifically, students' preference for online learning is most affected by their technical skills. Meanwhile, educators and administrators are influenced mainly by the perceived usefulness of online learning and practice conditions, respectively. We further conducted multi-group testing and confirmed the certain separation in online learning preferences of observed objects between the two countries. Overall, this paper enriches the literature on online education, and has important implications for educational policymakers and university stakeholders both during and after the pandemic.

What Shapes Undergraduate Students' Satisfaction in Unstable Learning Contexts?

This paper investigates what determinants, and to what extent, they influence students' satisfaction in unstable learning contexts. Using a national-scaled sample of Vietnamese HEIs with a sound theoretical background, we find that regardless of instabilities from external shocks, the key factors that shape students' satisfaction are fixed by traditional norms (self-efficacy, infrastructure, lecturer) rather than occasional factors occurring from each event. We find in particular that self-efficacy is the most influential factor for students' satisfaction and friendship is the most prominent element that enhances students' self- efficacy. Overall, this paper enriched the literature on student satisfaction, especially during unstable contexts. Thus, it has important implications for educators and HEIs stakeholders in management planning in the time to come.